Samuel H. Worthington was born around 1821 in Florida. He married Felicia Brown on January 14, 1845, and they had at least nine children: Mary, born around 1845; Eliza, born around 1847; Lucretia, born around 1849; Felicia, born around 1852; Stephen, born around 1855; Jane, born around 1856; James, born around 1857; Elizabeth, born around 1859; and Samuel, born around 1862. They lived in Columbia County, Florida, and he worked as a farmer. He moved to Levy County, Florida, in the 1850s, and by 1860, he owned $500 of real estate and $775 of personal property.
He enlisted in the Confederate army on April 5, 1862, and he mustered in as a private in the 6th Florida Infantry Battalion. The battalion took part in the Battle of Chickamauga and the Atlanta campaign. He was promoted to sergeant in December 1862.
In January 1864, he was court martialed for intoxication. The court declared him guilty and sentenced him “to be redused to ranks to serve as a Soldier for 25 days.” He was “wounded slightly” in February 1864, and he was promoted to 2nd lieutenant soon afterward. In June 1864, the battalion became part of the 9th Florida Infantry. He remained in the army until at least early 1865.
He returned to Levy County after the war, and he resumed his work as a farmer. By 1870, he owned $200 of real estate and $400 of personal property. He died on October 10, 1896.