Evelyn E. Moulthrop was born around 1841 in Connecticut to Elihu and Sarah Moulthrop. His father was a joiner. Moulthrop lived in Cheshire, Connecticut, until the 1850s, when he moved to Derby, Connecticut. By the early 1860s, he was working as a clock maker.
He enlisted in the Union army on August 9, 1862, and he mustered in as a private in Company B of the 20th Connecticut Infantry on September 8. The regiment took part in the Battle of Chancellorsville, the Battle of Gettysburg, and the Atlanta campaign. He was “accidentally killed” near Turner’s Ford, Georgia, on August 30, 1864.